Archive for March 28th, 2006

Refining the Genome

Church of Darwin, Science, Uncategorized | Posted by Chris Parker
Mar 28 2006
[22 October 2004]

Genome revised down to under 25,000 genes; failings of whole genome shotgun revealed

A comparison of human genome sequences produced using different approaches by the International Human Genome Sequencing Consortium and the private firm Celera reveals that whole genome shotgun sequencing (WGS), as used by Celera, fails miserably on genome segmental duplications.

Writing in Nature this week, Evan Eichler, from Washington University, and colleagues show that WGS does very well for 95% of euchromatin, but falls down in the very large duplications that are greater than 98% identical and longer than 20 kb in size.

“It’s not that bad unless you really care about those large identical duplications,” he told us. The technique “fails and fails miserably” in that regard, he said.

For the current study, Eichler teamed up with previous collaborators Granger G. Sutton and Aaron L. Halpern, both now at the J. Craig Venter Institute in Rockville, Md., in an apparent revisiting of results published by PNAS in February this year. They conclude that breaking up the sequencing of whole genomes into two phases – WGS followed by clone-order-based sequencing in bacterial artificial chromosomes – is the way forward.

Eichler told us he had wanted to return to the comparison because his work for the first paper was completed in just 2 weeks and had been reduced to three sentences of actual data in the PNAS paper. “At that point, I thought that I would do a much more rigorous analysis; that I’m going to broadcast this on my own,” he said.

Eichler and Sutton both felt Celera hadn’t published in more detail because of PNAS page constraints. But, said Halpern, “it wasn’t just page constraints that limited us back in February – we just didn’t have the results at the time.”

Eichler had only had time to do a preliminary analysis in the first place, Sutton and Halpern said. “So, for instance, in this [latest] one, the discussion is about the effect of the length of the repeat and the percent identity – those are entirely new to this paper,” Halpern said.

“The take-home message was that strict application of WGS is going to miss [segmental duplication] regions of the genome,” Eichler added. “In fact, it’s one of those things that without a complete finished sequence of the human genome, we would not understand the architecture of these regions.”

Those sentiments are echoed elsewhere in Nature this week, where Francis Collins, Adam Felsenfeld, and colleagues of the International Human Genome Sequencing Consortium report the final finishing of the human genome sequence.

“If we want to finish the genome sequences for other organisms besides the human, one cannot just count on the shotgun method to do that correctly, at least not in its current form,” Collins, director of the National Human Genome Research Institute (NHGRI), told us.

“That’s a lesson that we had pretty much resigned ourselves to,” Collins said. “Now that you have a really final beautifully finished sequence, you can compare it to that of other organisms, particularly the mouse, the rat, and the chimpanzee,” Collins said.

Still, it is the paucity of genes in the human genome – a figure revised downward to fewer than 25,000 – that continues to “blow our socks off,” according to Collins. It seems like an awfully short list to account for the biological properties of a human being, he said.

Felsenfeld, also at NHGRI, coordinated the teams involved in the sequencing project. He said the main surprises had only become apparent with the passage of time. “One of them is the real inhomogeneity of the genome. The most obvious manifestation of that is there is some small number of regions that we just can’t sequence,” he said.

“From here on out, it will require individual ingenuity to try and close any of those remaining gaps,” said Collins. But, he noted, there are probably few, if any, genes left in those unsequenceable regions.

First or Second Century B.C. Colima Toad, or Ankylosaur?

Crypto, Dinosaurs in Literature, Science, Uncategorized, Unexplained Artifact | Posted by Chris Parker
Mar 28 2006

Here at, we know that there’s probably no less exciting “dinosaur” than the ankylosaur —but here again we use this particular distinctive animal to prove that man and “dinosaur” co-existed, just as the Bible tells us.

Sure, we wish we had a first century example of a t-rex, but today we have to deal with what we have. (we do have more interesting examples on prior pages of this section)

The item shown above was for sale. The company selling it “knows” that ankylosaurs died out 65 million years ago, according to scientists who study such things. Therefore, this has to be a toad, (for which there is some resemblance, we suppose) which did still exist more than 2,500 years ago.

They describe it first as a toad and then later in the text as a “reptile”. Of course, toads are not reptiles, they are amphibians. Anklyosaurs were reptiles, however, but we’re probably just being picky.

Below we provide; the auctioneer’s full description, photos of the object in question, a picture of some actual toads, as well as a synopsis of what science says about the anklyosaur. You’ll have to decide for yourself if this is a Colima culture toad, –or a dinosaur.

Frankly, we think toads will be insulted by the comparison.

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